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src activity assay kits  (Millipore)

 
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    Millipore src activity assay kits
    Src Activity Assay Kits, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/src activity assay kits/product/Millipore
    Average 90 stars, based on 1 article reviews
    src activity assay kits - by Bioz Stars, 2026-04
    90/100 stars

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    A The effect of matrine on <t>Src</t> <t>kinase</t> <t>activity</t> in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity <t>detection</t> <t>kit.</t> Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.
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    A The effect of matrine on <t>Src</t> <t>kinase</t> <t>activity</t> in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity <t>detection</t> <t>kit.</t> Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.
    One Of The Src Activity Assay Kits, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    src tyrosine kinase activity assay kits  (Millipore)
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    Millipore src tyrosine kinase activity assay kits
    A The effect of matrine on <t>Src</t> <t>kinase</t> <t>activity</t> in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity <t>detection</t> <t>kit.</t> Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.
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    Image Search Results


    A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.

    Journal: Cell Death & Disease

    Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways

    doi: 10.1038/s41419-021-04221-6

    Figure Lengend Snippet: A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.

    Article Snippet: The activity of Src kinase was measured according to the instructions of Src kinase activity detection kit (GENMED Scientifics Inc., USA).

    Techniques: Activity Assay, Western Blot, Control

    Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.

    Journal: Cell Death & Disease

    Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways

    doi: 10.1038/s41419-021-04221-6

    Figure Lengend Snippet: Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.

    Article Snippet: The activity of Src kinase was measured according to the instructions of Src kinase activity detection kit (GENMED Scientifics Inc., USA).

    Techniques: Phospho-proteomics, Protein-Protein interactions, Activity Assay, Blocking Assay